Early recognized antigen (p34) of Toxoplasma gondii after peroral ingestion of tissue cyst forming strain (Me49 strain) in mice
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چکیده
منابع مشابه
Early recognized antigen (p34) of Toxoplasma gondii after peroral ingestion of tissue cyst forming strain (Me49 strain) in mice.
Serum from mouse orally ingested with tissue cyst forming strain (Me49) of Toxoplasma gondii was assayed by Western blot and immunofluorescene assay (IFA) to establish early responses in antigenicity of the parasite in mouse model of foodborne toxoplasmosis. Sera were collected weekly to blot the RH antigen transferred onto nitrocellulose paper after being separated by 12% SDS-PAGE. With the se...
متن کاملin vitro and in vivo potential of rh strain of toxoplasma gondii (type i) in tissue cyst forming.
background: based on recent studies, there are controversial reports on the ca-pacity of tissue cyst forming of toxoplasma gondii rh strain. in this study, the capac-ity was evaluated by in vivo and in vitro experiments. methods : rh strain was subcutaneously inoculated to ten wistar rats. after one month, their blood, brain, tongue and diaphragm were collected and evaluated by mat, pcr, pathol...
متن کاملIn Vitro and In Vivo Potential of RH Strain of Toxoplasma gondii (Type I) in Tissue Cyst Forming
BACKGROUND Based on recent studies, there are controversial reports on the capacity of tissue cyst forming of Toxoplasma gondii RH strain. In this study, the capacity was evaluated by in vivo and in vitro experiments. METHODS RH strain was subcutaneously inoculated to ten Wistar rats. After one month, their blood, brain, tongue and diaphragm were collected and evaluated by MAT, PCR, pathologi...
متن کاملPreparation of Toxoplasma gondii RH strain antigen, antigen analysis and antigen detection in sera: a review.
The prevalence of antibodies detected by serology against Toxoplasma gondii in Indonesia is quite high. Therefore further research concerning the antigen used is important to improve the quality of the assay being used with lower cost. An attempt to prepare T. gondii strain RH antigen followed by using it in the ELISA procedure for detecting IgG showed that there was no significant difference b...
متن کاملbrain tissue cysts in infected mice with rh-strain of toxoplasma gondii and evaluation of bag1 and sag1 genes expression.
background : toxoplasma gondii is an obligate intracellular parasite that infects humans at high preva-lence rates. the virulent rh strain of t. gondii is generally considered to have lost its cyst forming ca-pacity. this study performed to obtain tissue cysts in mice infected with tachyzoites of rh strain treated with sulfadiazine (sdz). it provides the opportunity to analyze the conversion of...
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ژورنال
عنوان ژورنال: The Korean Journal of Parasitology
سال: 1999
ISSN: 0023-4001
DOI: 10.3347/kjp.1999.37.3.157